Oligosaccharides isolated from goat milk reduce intestinal inflammation in a rat model of dextran sodium sulfate-induced colitis


      Background and aims

      There is increased interest in the study of manipulation of the flora with pro- and prebiotics regarding inflammatory bowel disease. The aim of this work was to evaluate the effect of oligosaccharides from goat milk in a rat model of dextran sodium sulfate- (DSS-) induced colitis.


      Twenty rats were fed the same diet but with different sources of fiber (5% of the diet): cellulose or a mixture of goat's milk oligosaccharides (GMO) and cellulose. DSS treatment was used to induce a colonic inflammation. Several clinical and inflammatory parameters, as well as intestinal micorbiota and gene expression by DNA microarray technology, were evaluated.


      DSS induced a decrease in body weight which was not observed in rats fed the GMO (decrease of 21±11% in control rats vs increase of 5.2±8.6 in GMO rats, P < 0.0 5 ). DSS also caused an acute colonic inflammatory process which was weaker in rats fed the GMO, as shown by colon myeloperoxidase activity (0.53±0.16 vs 0.14±0.07U/mg of protein, P < 0.0 5 ), as well as clinical symptoms measured by a scoring system (1.25±1.14 vs 0.4±0.07, P < 0.0 5 ). GMO rats also showed less severe colonic lesions and a more favorable intestinal microbiota. The expression of genes involved in intestinal function, such as mucine-3, was down-regulated in DSS-control rats but returned to normal values in GMO rats.


      GMO reduce intestinal inflammation and contribute to the recovery of damaged colonic mucosa.



      DSS (Dextran sodium sulfate), GMO (Goat's milk oligosaccharides), IBD (Inflammatory bowel disease), MPO (Myeloperoxidase), PF (Pair-fed), SCFA (Short-chain fatty acids)
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        • Riddell R.H.
        Pathology of idiopathic inflammatory bowel diseases.
        in: Kirsner J.B. Shorter R.G. Inflammatory bowel diseases. Lea and Febiger, Philadelphia1998: 329-350
        • Lauritsen K.
        Drug treatment and formation of eicosanoids in patients with chronic inflammatory bowel diseases.
        Dan Med Bull. 1989; 36: 378-393
        • Elson C.
        • et al.
        Experimental models of inflammatory bowel disease.
        Gastroenterology. 1995; 109: 1344-1367
        • Shanahan F.
        Probiotics in inflammatory bowel disease—therapeutic rationale and role.
        Adv Drug Deliver Rev. 2004; 56: 809-818
        • Chandran P.
        • Satthaporn S.
        • Robins A.
        • Eremin O.
        Inflammatory bowel disease: dysfunction of GALT and gut bacterial flora (II).
        Surgeon. 2003; 1: 125-136
        • Osman N.
        • Adawi D.
        • Ahrne S.
        • Jeppsson B.
        • Molin G.
        Modulation of the effect of dextran sulfate sodium-induced acute colitis by the administration of different probiotic strains of Lactobacillus and Bifidobacterium.
        Dig Dis Sci. 2004; 49: 320-327
        • Araki Y.
        • Andoh A.
        • Takizawa J.
        • Takizawa W.
        • Fujiyama Y.
        Clostridium butyricum, a probiotic derivative, suppresses detran sulfate sodium-induced experimental colitis in rats.
        Int J Mol Med. 2004; 13: 577-580
        • Rumi G.
        • Tsubouchi R.
        • Okayama M.
        • Kato S.
        • Mozsik G.
        • Takeuchi K.
        Protective effect of lactulose on dextran sulphate sodium-induced colonic inflammation in rats.
        Dig Dis Sci. 2004; 49: 1466-1472
        • Kanauchi O.
        • Andoh A.
        • Iwanaga T.
        • et al.
        Germinated barley foodstuffs attenuate colonic mucosal damage and mucosal nuclear factor κB in a spontaneous colitis model.
        J Gastroenterol Hepatol. 1999; 14: 1173-1179
        • Kunz C.
        • Rudloff S.
        • Baier W.
        • et al.
        Oligosaccharides in human milk: structural, functional and metabolic aspects.
        Annu Rev Nutr. 2000; 20: 699-722
        • Mc Guire W.
        • Anthony M.Y.
        Donor human milk versus formula for preventing necrotising enterocolitis in preterm infants: systematic review.
        Arch Dis Child Fetal Neonatal. 2003; 88: F11-F14
        • Martínez-Férez A.
        • Rudolff S.
        • Guadix A.
        • et al.
        Goat's milk as a natural source of lactose-derived oligosaccharides: isolation by membrane technology.
        Int Dairy J. 2005; 16: 173-181
        • Okayasu I.
        • Hatakeyama S.
        • Yamada M.
        • Ohkusa T.
        • Inagaki Y.
        • Nakaya R.
        A novel method in the induction of reliable experimental acute and chronic ulcerative colitis in mice.
        Gastroenterology. 1990; 98: 694-702
        • Mercier S.
        • Breuillé D.
        • Mosoni L.
        • et al.
        Chronic inflammation alters protein metabolism in several organs of adult rats.
        J Nutr. 2002; 132: 1921-1928
        • Stucchi A.F.
        • Shofer S.
        • Leeman S.
        • et al.
        NK-1 antagonist reduces colonic inflammation and oxidative stress in dextran sulfate sodium colitis in rats.
        Am J Physiol. 2000; 279: G1298-G1306
        • Iwai A.
        • Iwashita E.
        Changes in colonic inflammation induced by dextran sulphate sodium (DSS) during short- and long-term administration of rebamipide.
        Dig Dis Sci. 1998; 43: 143S-147S
        • Krawisz J.E.
        • Sharon P.
        • Stenson W.F.
        Quantitative assay for acute intestinal inflammation in rat and hamster models.
        Gastroenterology. 1984; 84: 1344-1350
        • Martin J.
        • White I.N.H.
        Fluorimetric determination of oxidised and reduced glutathione in cells and tissues by high-performance liquid chromatography following derivatization with dansyl chloride.
        J Chromatogr B Biomed Appl. 1991; 568: 219-225
        • Araki Y.
        • Andoh A.
        • Tsujikawa T.
        • et al.
        Alterations in intestinal microflora, faecal bile acids and short chain fatty acids in dextran sulphate sodium-induced experimental acute colitis in rats.
        Eur J Gastroenterol Hepatol. 2001; 13: 107-111
        • Mortensen P.B.
        • Clausen M.R.
        Short-chain fatty acids in the human colon: relation to gastrointestinal health and disease.
        Scand J Gastroenterol. 1996; 31: 132S-148S
        • Hoffmann P.
        • Reinshagen M.
        • Zeeh J.M.
        • et al.
        Increased expression of epidermal growth factor-receptor in an experimental model of colitis in rats.
        Scand J Gastroenterol. 2000; 35: 1174-1180
        • Spisni E.
        • Manica F.
        • Tomasi V.
        Involvement of prostanoids in the regulation of angiogenesis by polypeptide growth factors.
        Prostaglandins Leukot Essent Fatty Acids. 1992; 47: 111-115
        • Sanchez de Medina F.
        • Martinez-Augustin O.
        • Gonzalez R.
        • et al.
        Induction of alkaline phosphatase in the inflamed intestine: a novel pharmacological target for inflammatory bowel disease.
        Biochem Pharmacol. 2004; 68: 2317-2326
        • Guarner F.
        • Malagelada J.R.
        Role of bacteria in experimental colitis.
        Best Pract Res Clin Gastroenterol. 2003; 17: 793-804
        • Perán L.
        • Camuesco D.
        • Comalada M.
        • et al.
        Preventive effects of a probiotic Lactobacllus salivarius ssp.sallivarius in the TNBS model of rat colitis.
        World J Gastroenterol. 2005; 11: 5185-5192
        • Otte J.M.
        • Podolsky D.K.
        Functional modulation of enterocytes by gram-positive and gram-negative microorganisms.
        Am J Physiol Gastrointest Liver Physiol. 2004; 286: G613-G626
        • Videla S.
        • Vilaseca J.
        • Antolin M.
        • et al.
        Dietary inulin improves distal colitis induced by dextran sodium sulfate in the rat.
        Am J Gastroenterol. 2001; 96: 1486-1493
        • Moreau N.M.
        • Martin L.J.
        • Toquet C.S.
        • et al.
        Restoration of the integrity of rat caeco-colonic mucosa by resistant starch, but not by fructo-oligosaccharides, in dextran sulphate sodium-induced experimental colitis.
        Br J Nutr. 2003; 90: 75-85
        • Rodriguez-Cabezas M.E.
        • Galvez J.
        • Lorente M.D.
        • et al.
        Dietary fiber down-regulates Colonia tumor necrosis factor α in trinitrobenzenesulfonic acid-induced Colitic rats.
        J Nutr. 2002; 132: 3263-3271
        • Cherbut C.
        • Michel C.
        • Lecannu G.
        The prebiotic characteristics of fructooligosaccharides are necessary for reduction of TNBS-induced colitis in rats.
        J Nutr. 2003; 133: 21-27
        • Newburg D.S.
        Oligosaccharides in human milk and bacterial colonization.
        J Pediatr Gastroenterol Nutr. 2000; 30: S8-S17
        • Faure M.
        • Moennoz D.
        • Mettraux C.
        • et al.
        The chronic colitis developed by HLA-B27 transgenic rats is associated with altered in vivo mucin synthesis.
        Dig Dis Sci. 2004; 49: 339-346
        • Faure M.
        • Moënoz D.
        • Montignon F.
        • et al.
        Mucin production and composition is altered in dextran sulfate sodium-induced colitis rats.
        Dig Dis Sci. 2003; 48: 1366-1373
        • Renes I.B.
        • Boshuizen J.A.
        • Van Nispen D.J.
        • et al.
        Alterations in Muc2 biosynthesis and secretion during dextran sulfate sodium-induced colitis.
        Am J Physiol Gastrointest Liver Physiol. 2001; 282: G382-G389
        • Bode L.
        • Kunz C.
        • Muhly Reinholz M.
        • et al.
        Inhibition of monocyte, lymphocyte and neutrophil adhesion to endothelial cells by human milk oligosaccharides.
        Thromb Haemost. 2004; 92: 1402-1410