Relationship of omega-3 and omega-6 fatty acids with semen characteristics, and anti-oxidant status of seminal plasma: A comparison between fertile and infertile men


      Background & aims

      Fatty acid (FA) composition of the spermatozoa may be an important determinant of fertility. The aim was to evaluate polyunsaturated fatty acid (PUFA) composition of the blood plasma and spermatozoa in infertile men with idiopathic oligoasthenoteratozoospermia (OAT).


      Eighty-two infertile men with idiopathic OAT and seventy-eight fertile men defined according to semen concentration and proven fertility were enrolled in the study. The semen parameters were assessed according to World Health Organization criteria; three omega-3 fatty acids – α-linolenic acid (ALA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and two omega-6 fatty acids – linoleic acid (LA) and arachidonic acid (AA) concentrations were measured in blood plasma and spermatozoa; and the seminal plasma enzymatic antioxidant levels of catalase, and superoxide dismutase (SOD) were also assessed.


      Proven fertile men had higher blood and spermatozoa levels of omega-3 FAs compared with the infertile patients. The ratio of serum omega-6/omega-3 fatty acids was significantly higher in infertile (14.8±4.3) patients compared to fertile controls (6.3±2.2) (P=0.001). Additionally, levels of AA were higher and the omega-3 index (EPA+DHA) was lower in infertile subjects than in fertile controls (all P values<0.05). Infertile men had higher mean AA:DHA ratio and AA:EPA (6.4±2.9 and 12.0±4.9, respectively) than fertile men (3.3±1.8 and 6.7±2.6, respectively) (both P=0.001). A strong negative correlation was found between the AA:DHA and AA:EPA ratios and total sperm count (r=−0.62, P=0.001 and r=−0.64, P=0.001, respectively), sperm motility (r=−0.63, P=0.001 and r=−0.61, P=0.001, respectively), and sperm morphology (r=−0.61, P=0.001, and r=−0.59, P=0.002, respectively).


      Infertile men had lower concentrations of omega-3 FAs in spermatozoa than fertile men. These results suggest that research should be performed to assess the potential benefits of omega-3 FA supplementation as a therapeutic approach in infertile men with idiopathic OAT.


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        • Templeton A.
        Infertility—epidemiology, etiology and effective management.
        Health Bull. 1995; 53: 294-298
        • Safarinejad M.R.
        Sperm DNA damage and semen quality impairment after treatment with selective serotonin reuptake inhibitors detected using semen analysis and sperm chromatin structure assay.
        J Urol. 2008; 180: 2124-2128
        • Safarinejad M.R.
        Infertility among couples in a population-based study in Iran: prevalence and associated risk factors.
        Int J Androl. 2008; 31: 303-314
        • Carlsen E.
        • Giwercman A.
        • Keiding N.
        • Skakkebaek N.E.
        Evidence for decreasing quality of semen during past 50 years.
        BMJ. 1992; 305: 609-613
        • Fraser L.R.
        New insights into possible causes of male infertility.
        Humanit Rep. 1999; 14: 38-46
        • Farooqui A.A.
        • Horrocks L.A.
        • Farooqui T.
        Glycerophospholipids in brain: their metabolism, incorporation into membranes, functions, and involvement in neurological disorders.
        Chem Phys Lipids. 2000; 106: 1-29
        • Lenzi A.
        • Gandini L.
        • Maresca V.
        • Rago R.
        • Sgrò P.
        • Dondero F.
        • et al.
        Fatty acid composition of spermatozoa and immature germ cells.
        Mol Hum Reprod. 2000; 6: 226-231
        • Zalata A.A.
        • Christophe A.B.
        • Depuydt C.E.
        • Schoonjans F.
        • Comhaire F.H.
        The fatty acid composition of phospholipids of spermatozoa from infertile patients.
        Mol Hum Reprod. 1998; 4: 111-118
        • Conquer J.A.
        • Martin J.B.
        • Tummon I.
        • Watson L.
        • Tekpetey F.
        Fatty acid analysis of blood serum, seminal plasma, and spermatozoa of normozoospermic vs. asthenozoospermic males.
        Lipids. 1999; 34: 793-799
        • Calamera J.
        • Buffone M.
        • Ollero M.
        • Alvarez J.
        • Doncel G.F.
        Superoxide dismutase content and fatty acid composition in subsets of human spermatozoa from normozoospermic, asthenozoospermic, and polyzoospermic semen samples.
        Mol Reprod Dev. 2003; 66: 422-430
        • Agarwal A.
        • Saleh R.A.
        • Bedaiwy M.A.
        Role of reactive oxygen species in the pathophysiology of human reproduction.
        Fertil Steril. 2003; 79: 829
        • Xu D.X.
        • Shen H.M.
        • Zhu Q.X.
        • Chua L.
        • Wang Q.N.
        • Chia S.E.
        • et al.
        The associations among semen quality, oxidative DNA damage in human spermatozoa and concentrations of cadmium, lead and selenium in seminal plasma.
        Mutat Res. 2003; 534: 155-163
        • Hodge W.G.
        • Schachter H.M.
        • Barnes D.
        • Pan Y.
        • Lowcock E.C.
        • Zhang L.
        • et al.
        Efficacy of omega-3 fatty acids in preventing age-related macular degeneration: a systematic review.
        Ophthalmology. 2006; 113: 1165-1172
        • Mazza M.
        • Pomponi M.
        • Janiri L.
        • Bria P.
        • Mazza S.
        Omega-3 fatty acids and antioxidants in neurological and psychiatric diseases: an overview.
        Prog Neuropsychopharmacol Biol Psychiatry. 2007; 31: 12-26
        • World Health Organization
        WHO laboratory manual for the examination of human sperm and sperm–cervical mucus interaction.
        3rd ed. Cambridge University Press, Cambridge1992
        • Imade G.E.
        • Baker H.W.
        • de Kretser D.M.
        • Hedger M.P.
        Immunosuppressive activities in the seminal plasma of infertile men: relationship to sperm antibodies and autoimmunity.
        Hum Reprod. 1997; 12: 256-262
        • Ma J.
        • Folsom A.R.
        • Shahar E.
        • Eckfeldt J.H.
        Plasma fatty acid composition as an indicator of habitual dietary fat intake in middle-aged adults.
        Am J Clin Nutr. 1995; 62: 564
        • Zini A.
        • de Lamirande E.
        • Gagnon C.
        Reactive oxygen species in the semen of infertile patients: levels of superoxide dismutase- and catalase-like activities in seminal plasma and spermatozoa.
        Int J Androl. 1993; 16: 183
        • Giraud M.N.
        • Motta C.
        • Boucher D.
        • Grizard G.
        Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa.
        Hum Reprod. 2000; 15: 2160-2164
        • Abayasekara D.R.E.
        • Wathes D.C.
        Effects of altering dietary fatty acid composition on prostaglandin synthesis and fertility.
        Prostaglandins Leukot Essent Fatty Acids. 1999; 61: 275-287
        • Comhaire F.H.
        • Christophe A.B.
        • Zalata A.A.
        • Dhooge W.S.
        • Mahmoud A.M.
        • Depuydt C.E.
        The effects of combined conventional treatment, oral antioxidants and essential fatty acids on sperm biology in subfertile men.
        Prostaglandins Leukot Essent Fatty Acids. 2000; 63: 159-165
        • Khosrowbeygi A.
        • Zarghami N.
        Fatty acid composition of human spermatozoa and seminal plasma levels of oxidative stress biomarkers in subfertile males.
        Prostaglandins Leukot Essent Fatty Acids. 2007; 77: 117-121
        • Lenzi A.
        • Gandini L.
        • Picardo M.
        • Tramer F.
        • Sandri G.
        • Panfili E.
        Lipoperoxidation damage of spermatozoa polyunsaturated fatty acids (PUFA): scavenger mechanisms and possible scavenger therapies.
        Front Biosci. 2000; 5: E1-E15
        • Gulaya N.M.
        • Margitich V.M.
        • Govseeva N.M.
        • Klimashevsky V.M.
        • Gorpynchenko II,
        • Boyko M.I.
        Phospholipid composition of human sperm and seminal plasma in relation to sperm fertility.
        Arch Androl. 2001; 46: 169-175
        • Mcelhaney R.N.
        • de Gier J.
        • van der Neut-Kok E.C.
        The effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of Acholeplasma laidlawii B cells and derived liposomes.
        Biochim Biophys Acta. 1973; 298: 500-512
        • Lyons J.M.
        • Raison J.K.
        • Kumamoto J.
        Polarographic determination of phase changes in mitochondrial membranes in response to temperature.
        Meth Enzymol. 1974; 32: 258-262
        • Aitken R.J.
        • Krausz C.
        Oxidative stress, DNA damage and the Y chromosome.
        Reproduction. 2001; 122: 497-506
        • Turnbull T.
        • Cullen-Drill M.
        • Smaldone A.
        Efficacy of omega-3 fatty acid supplementation on improvement of bipolar symptoms: a systematic review.
        Arch Psychiatr Nurs. 2008; 22: 305-311
        • Wathes D.C.
        • Abayasekara D.R.E.
        • Aitken R.J.
        Polyunsaturated fatty acids in male and female reproduction.
        Biol Reprod. 2007; 77: 190